T8535
Sigma
Monoclonal Anti-β-Tubulin I+II antibody produced in mouse
clone JDR.3B8, ascites fluid
| MDL number: | MFCD00145892 |
Description
| Immunogen | synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype II coupled to BSA |
| Specificity | The antibody specifically recognizes an epitope located on isotypes I and II of β-tubulin. No cross-reactivity with other tubulin isotypes is observed. |
Properties
| antibody form | ascites fluid |
| clone | JDR.3B8 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | pig, mouse, rat, chicken, human, bovine |
| application(s) | immunoblotting: 1:1,000 using a chicken fibroblast extract or a chicken brain microtubule proteins preparation |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| microarray: suitable | |
| isotype | IgG2b |
| Gene Information | human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) |
| rat ... Tubb2(29212), Tubb2c(296554) | |
| mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Banerjee, A., et al., A monoclonal antibody against the type II isotype of β-tubulin. Preparation of isotypically altered tubulin. J. Biol. Chem. 263, 3029, (1988) Abstract |
| Banerjee, A., et al., Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of β-tubulin. J. Biol. Chem. 265, 1794, (1990) Abstract | |
| Moustakas, A., et al., Id2 and Id3 Define the Potency of Cell Proliferation and Differentiation Responses to Transforming Growth Factor β and Bone Morphogenetic Protein Mol. Cell. Biol. 24, 4241-4254, (2004) Abstract | |
| Banerjee, A., et al., Direct involvement of the isotype-specific C-terminus of β-tubulin in ciliary beating J. Cell Sci. 118, 4333-4341, (2005) Abstract | |
| Amaya, E., et al., BDNF promotes target innervation of Xenopus mandibular trigeminal axons in vivo BMC Dev. Biol. 7, 59, (2007) Abstract |
